Prevalence of blaCTX-M, blaCTX-M-2, blaCTX-M-8,
blaCTX-M-25 and blaCTX-M-3 Genes in Escherichia
coli Isolated from Urinary Tract Infection in
Kermanshah City, Iran
Published: August 1, 2019 | DOI: https://doi.org/10.7860/JCDR/2019/40684.13055
Alisha Akya, Mahnaz Ahmadi, Sepideh Khodamoradi, Mohammad Reza Rezaei, Nahid Karani, Azam Elahi, Roya Chegene Lorestani, Mansour Rezaei
1. Associate Professor, Department of Medical Microbiology, Infectious Diseases Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
2. MSc in Medical Surgical Nursing, Imam Reza Hospital, Kermanshah University of Medical Sciences, Kermanshah, Iran.
3. PhD, Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Shahr-e-Qods Branch, Tehran, Iran.
4. Associate Professor, Department of Emergency Medicine, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.
5. BSc in Nursing, Imam Reza Hospital, Kermanshah University of Medical Sciences, Kermanshah, Iran.
6. MSc in Medical Microbiology, Infectious Diseases Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
7. MSc in Medical Microbiology, Infectious Diseases Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
8. Associate Professor, Department of Biostatistics, School of Health, Kermanshah University of Medic
Correspondence
Dr. Mansour Rezaei,
School of Health, Kermanshah University of Medical Sciences, Kermanshah, Iran.
E-mail: mansourreza05@gmail.com
Introduction: Urinary Tract Infection (UTI) is one of the most common bacterial infections and Escherichia coli is the most common organism that causes UTI. However, the incidence of community acquired UTI caused by Extended spectrum beta-lactamase (ESBL)-producing strains of E.coli, in particular CTX-M genes, is on the rise worldwide.
Aim: To detect the frequency of CTX-M gene subgroups in uropathogenic E.coli.
Materials and Methods: In this descriptive-analytical study, 240 isolates of E. coli were studied. All isolates were isolated from UTIs in Kermanshah University of Medical Sciences, Kermanshah, Iran, in 2014 to 2015. After screening for ESBL, the CTX-M, CTX-M-2, CTX-M-8, CTX-M-25 and CTX-M-3 genes were detected among ESBL- producing isolates using PCR.
Results: Of the 240 E. coli isolates, 67 were ESBL-producing isolates. Sixty one isolates (91%) contained CTX-M gene, of which 57 (85%), 3 (4.5%), 3 (4.5%) and 1(1.49%) contained CTX-M-3, CTX-M-8, CTX- M-25 and CTX-M-2, respectively.
Conclusion: Due to the high resistance of E. coli to beta-lactam drugs in this region, these drugs have limited effects for treatment of UTI in outpatient. The frequency of CTX-M-2, CTX-M-8, CTX-M-25 beta-lactamases in isolates of E. coli is relatively low but the overall prevalence of CTX-M and CTX-M-3 beta-lactamases is high which indicates the spread of drug resistance.
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